Anoxic MBBR

Denitrification on biofilm carriers under oxygen-free, nitrate-rich conditions.

In an anoxic MBBR the reactor is kept free of dissolved oxygen so that denitrifying bacteria on the carriers use nitrate (or nitrite) as the electron acceptor instead, converting it to harmless nitrogen gas. This is the biofilm equivalent of the anoxic zone in a conventional denitrification process, but without suspended sludge and without sludge return.

Process principles

Denitrification requires an organic carbon source as electron donor and nitrate (or nitrite) as electron acceptor, in the absence of dissolved oxygen — because oxygen is preferred and suppresses nitrate use. In wastewater the carbon can come from the influent BOD (pre-denitrification layout, where the anoxic stage is before the aerobic stage) or from an external source such as methanol or acetate (post-denitrification).

Because there is no aeration, mechanical mixers keep the carriers circulating. The mixer must be powerful enough to fluidise the media without entraining air from the surface, which would raise the dissolved oxygen and inhibit denitrification.

Key design factors

Anoxic MBBR design factors (indicative — confirm per project)

ParameterTypical ValueUnitNotes
Electron acceptorNitrate / nitrite—Dissolved oxygen must be absent.
Carbon sourceInfluent BOD or external—Pre-DN uses influent; post-DN needs dosing.
MixingMechanical—Must avoid surface air entrainment.
LayoutPre- or post-anoxic—Position determines carbon availability.

Frequently Asked Questions

Why must the anoxic reactor be free of dissolved oxygen?
Bacteria preferentially use dissolved oxygen over nitrate. Even small amounts of DO suppress denitrification, so the reactor must be oxygen-free and the mixer must not entrain air from the surface.
Does anoxic MBBR need a carbon source?
Yes. Denitrifying bacteria need organic carbon as an electron donor. In a pre-anoxic layout the influent wastewater supplies the carbon; in a post-anoxic layout an external carbon source (e.g. methanol, acetate) is typically dosed.
How are carriers mixed without aeration?
Mechanical mixers (slow-speed submersible mixers or similar) keep the carriers circulating. The mixer design must avoid surface vortex and air entrainment to maintain true anoxic conditions.

Related Pages

Need MBBR Design Support?

Submit your wastewater data for a preliminary MBBR engineering assessment. Our team reviews every submission within 2 business days.

Start MBBR Assessment