Excessive Biofilm Growth in MBBR

When carriers become overloaded with biomass — causes, consequences and control.

While biofilm is essential for MBBR function, too much biofilm is counterproductive. Excessively thick biofilm develops anaerobic inner layers that are biologically inactive, clogs carrier channels, increases carrier weight (impairing mixing), and can cause screening and downstream solids problems. The goal is an active, thin biofilm — not maximum biofilm.

Why excessive biofilm develops

High organic loading: A heavily loaded first-stage reactor promotes rapid heterotrophic growth that can outpace natural detachment. Low shear: Insufficient aeration or mixing energy means the erosion force is not strong enough to keep biofilm in check. Protected carrier geometry: Carriers with deep internal channels protect biofilm from shear, which is an advantage up to a point but can lead to channel clogging under high load.

Filamentous bacteria: In some cases, filamentous organisms colonise the biofilm and create a thick, slimy or hairy layer that traps solids and is resistant to normal erosion. This is analogous to filamentous bulking in activated sludge and may be triggered by low DO, nutrient deficiency, or specific substrate conditions.

Consequences and indicators

Excessive biofilm: signs and effects

ParameterTypical ValueUnitNotes
Carrier channels cloggedReduced protected surface area—Pull carriers and inspect.
Carriers sinking / raftingIncreased density or gas entrapment—Mixing energy insufficient.
Effluent TSS spikeSloughing of thick biofilm—Downstream clarifier overloaded.
Reduced nitrificationO₂ cannot reach nitrifiers—Thick heterotrophic layer blocks O₂.
Screen cloggingDetached biofilm blocks screens—Increased backwash frequency.

Control measures

Increase aeration / mixing energy: Higher shear promotes erosion and thins the biofilm. This is the primary control measure. Redistribute load: If the first stage is overloaded, divert some flow to later stages or activate standby volume to reduce the surface-area loading. Temporary air scouring: A short burst of high-intensity aeration can strip excess biofilm; this should be done carefully to avoid losing too much biomass.

In persistent cases, physical cleaning of carriers (removing and washing) has been used, but this is labour-intensive and disruptive. Preventing excessive growth through proper load management and aeration is always preferable to reactive cleaning.

Frequently Asked Questions

How thick is "too thick"?
As a rough guide, biofilm above 500–1 000 µm is likely too thick for efficient treatment. However, the best indicator is performance: if treatment efficiency is declining despite adequate DO and the carriers visually show thick, dark or slimy biofilm, it is excessive. Inner layers are anaerobic and contribute nothing to aerobic treatment.
Will increasing aeration strip all the biofilm?
If the increase is moderate and sustained, it will gradually thin the biofilm to a new equilibrium without stripping it completely. A sudden, large increase could cause excessive detachment. Ramp up aeration over a few days and monitor effluent TSS and treatment performance.
Can filamentous biofilm be controlled?
The approach mirrors filamentous control in activated sludge: identify the cause (often low DO, nutrient deficiency or specific substrates), correct it, and allow the microbial community to shift. Increasing DO and ensuring adequate N and P are the most common corrective actions. Chemical treatment (e.g. chlorination) is a last resort and risks damaging the desirable biofilm as well.

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